| Enzyme | Function | |--------|----------| | | Unwinds and separates the double helix by breaking hydrogen bonds. | | DNA Polymerase III | Synthesizes new DNA strands by adding nucleotides to the 3’ end; also has 3’→5’ proofreading ability. | | Primase | Synthesizes a short RNA primer to provide a 3’-OH group for DNA polymerase to begin adding nucleotides. | | Ligase | Seals nicks (joins Okazaki fragments) on the lagging strand by forming phosphodiester bonds. | | Topoisomerase | Relieves supercoiling ahead of the replication fork to prevent DNA breakage. |
If one strand of DNA has the sequence 5’-ATTCGAGC-3’, what is the complementary strand? Be sure to label the 5’ and 3’ ends. Dna Structure And Function Worksheet Ap Biology Answer
The purine-to-pyrimidine ratio is always 1:1 in a double-stranded DNA molecule (Chargaff’s rule). Part 2: DNA Function (Replication & Central Dogma) Question 5: Explain the role of the following enzymes in DNA replication: | Enzyme | Function | |--------|----------| | |
Hydrogen bonds hold the two strands together. Specifically, two hydrogen bonds form between A and T, and three hydrogen bonds form between G and C. | | Ligase | Seals nicks (joins Okazaki
What type of bond holds the two strands of DNA together? Why is this bond important for DNA replication?